| Person | Type | Job | Role(s) | Episodes |
|---|---|---|---|---|
 Eric S. Rollman | Crew | Production | Executive Producer | 26 |
Greg Weisman | Crew | Writing | Writer | 1 |
Jamie Thomason | Crew | Production | Casting | 26 |
 Jay Oliva | Crew | Writing | Storyboard | 2 |
 Kevin Altieri | Crew | Directing | Director | 2 |
Kristopher Carter | Crew | Sound | Original Music Composer | 26 |
Lolita Ritmanis | Crew | Sound | Original Music Composer | 26 |
Michael McCuistion | Crew | Sound | Original Music Composer | 26 |
 Stan Lee | Crew | Production | Executive Producer, Creator | 26 |
 Steve Ditko | Crew | Creator | Creator | |
 Victor Cook | Crew | Directing | Director | 1 |
 Craig Kyle | Crew | Production | Executive Producer | 26 |
Heiko von Drengenberg | Crew | Writing | Storyboard | 1 |
 José Zelaya | Crew | Visual Effects | Character Designer | 26 |
Joshua Fine | Crew | Production | Executive Producer | 26 |
 Alan Rachins | Cast | Actor | Norman Osborn (voice) | 15 |
 Alanna Ubach | Cast | Actress | Liz Allan / Helena (voice) | 1 |
 Ben Diskin | Cast | Actor | Venom (voice) | 3 |
 Clancy Brown | Cast | Actor | Rhino / George Stacy (voice) | 3 |
 Cree Summer | Cast | Actress | Glory Grant / Cobweb / Homunculus #2 (voice) | 1 |
 Crispin Freeman | Cast | Actor | Electro / Clerk (voice) | 1 |
 Danny Trejo | Cast | Actor | Ox (voice) | 1 |
 Daran Norris | Cast | Actor | J. Jonah Jameson / John Jameson (voice) | 2 |
 Deborah Strang | Cast | Actress | May Parker (voice) | 16 |
 Dee Bradley Baker | Cast | Actor | Curt Connors / Homunculi (voice) | 1 |
 Ed Asner | Cast | Actor | Ben Parker (voice) | 1 |
Eric Vesbit | Cast | Kraven (voice) | 1 | |
 Grey DeLisle | Cast | Actress | Sally Avril / Voice of OsCorp (voice) | 1 |
 James Arnold Taylor | Cast | Actor | Harry Osborn / Alan O'Neil (voice) | 1 |
 John DiMaggio | Cast | Actor | Sandman (voice) | 1 |
 Josh Keaton | Cast | Actor | Peter Parker / Spider Man (voice) | 26 |
 Joshua LeBar | Cast | Actor | Flash Thompson / Car Thief #1 (voice) | 1 |
 Kevin Michael Richardson | Cast | Actor | OsCorp Security Guard (voice) | 1 |
 Lacey Chabert | Cast | Actress | Gwen Stacy (voice) | 24 |
 Miguel Ferrer | Cast | Actor | Silvio Manfredi / Silvermane (voice) | 1 |
 Peter MacNicol | Cast | Actor | Master Planner / Doctor Octopus (voice) | 1 |
 Phil LaMarr | Cast | Actor | Fancy Dan / Homunculus #1 (voice) | 1 |
 Robert Englund | Cast | Actor | Vulture (voice) | 3 |
Stan Lee | Cast | Actor | Stan (voice) | 1 |
 Tricia Helfer | Cast | Actress | Felicia Hardy / Black Cat (voice) | 1 |
 Vanessa Marshall | Cast | Actress | Mary Jane Watson / Hermia (voice) | 1 |
| Person | Type | Job | Role(s) | Episodes |
